Open AccessRapid synthesis of oligodeoxyribonucleotides VI. Effident, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route
Author(s) -
Mary Lynn Duckworth,
Michael J. Gait,
Philip Goelet,
Guo Hong,
Mandeep Singh,
Richard C. Titmas
Publication year - 1981
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/9.7.1691
Subject(s) - oligonucleotide , biology , combinatorial chemistry , oligonucleotide synthesis , solid phase synthesis , dna , dna synthesis , phase (matter) , nucleic acid , biochemistry , organic chemistry , chemistry , peptide
Efficient mechanised synthesis of heptadecadeoxyribonucleotides has been achieved on an economically small scale by an improved solid phase phosphotriester method on a polydimethylacrylamide resin. Improvements were made in the preparation of dinucleotide building blocks, reaction conditions for oligonucleotide assembly and in purification of deprotected oligonucleotides by h.p.l.c. Several milligrams of pure heptadecamers were obtained. Two of the heptadecamers were designed for sequencing in opposite directions of DNA cloned in phage M13mp2.