In vitro transcription of a cloned mouse nbosomal RNA gene | Zendy

Yukio Mishima | Zendy; Osami Yamamoto | Zendy; Ryo Kominami | Zendy; Masami Muramatsu | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

In vitro transcription of a cloned mouse nbosomal RNA gene

Author(s) -

Yukio Mishima,

Osami Yamamoto,

Ryo Kominami,

Masami Muramatsu

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.24.6773

Subject(s) - biology , transcription (linguistics) , gene , rna , genetics , in vitro , microbiology and biotechnology , philosophy , linguistics

An in vitro transcription system which utilizes cloned mouse ribosomal RNA gene (rDNA) fragments and a mouse cell extract has been developed. RNA polymerases I is apparently responsible for this transcription as evidenced by the complete resistance to a high concentration (200 micrograms/ml) of alpha-amanitin. Run-off products obtained with three different truncated rDNA fragments indicated that RNA was transcribed from a unique site of rDNA. The S1 nuclease protection mapping of the in vitro product and of in vivo 45S RNA confirmed this site, indicating that, in this in vitro system, transcription of rDNA started from the same site as in vivo. This site is located at several hundred nucleotides upstream from the putative initiation site reported by us (1) and by others (2). Some sequence homology surrounding this region was noted among mouse, Xenopus laevis and Drosophila melanogaster. The data also suggest that some processing of the primary transcript occurs in this in vitro system.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research