Open AccessSelective in vitro transcription of one of the two Alu family repeats present in the 5′ flanking region of the human ε-globin gene
Author(s) -
Gianfranco Di Segni,
G. Carrara,
Guiseppe TocchiniValentini,
Carol C. Shoulders,
Francisco E. Baralle
Publication year - 1981
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/9.24.6709
Subject(s) - biology , genetics , 5' flanking region , gene , alu element , globin , transcription (linguistics) , repeated sequence , in vitro , gene family , intron , promoter , microbiology and biotechnology , human genome , gene expression , genome , linguistics , philosophy
The sequence of 2965 nucleotides 5' of the human epsilon-globin gene has been completed. It includes two Alu family repeats present in an inverted configuration. Only the one located farthest from the gene was active as template for RNA polymerase III in a transcription system prepared from nuclei of Xenopus laevis oocytes. This selective transcription may be explained by the lack of homology of the first 45 nucleotides of the non transcribed repeat with other members of the Alu family. In fact this region includes one of the homology blocks described for other RNA polymerase III templates.