Isolation and characterization of cloned rat DNA fragments carrying tRNA genes | Zendy

M. Lasser-Weiss | Zendy; Nurit Bawnik | Zendy; Ada Rosen | Zendy; S Sarid | Zendy; V. Daniel | Zendy

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Isolation and characterization of cloned rat DNA fragments carrying tRNA genes

Author(s) -

M. Lasser-Weiss,

Nurit Bawnik,

Ada Rosen,

S Sarid,

V. Daniel

Publication year - 1981

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/9.22.5965

Subject(s) - biology , transfer rna , gene , genetics , xenopus , dna , rna , microbiology and biotechnology , genome , genomic library , peptide sequence

A rat genomic library was screened for tRNA genes with an unfractionated rat liver tRNA probe. About 70 clones containing tRNA genes were detected per rat genome. The organization of tRNA genes in five clones was analyzed by restriction endonuclease digestion, RNA-DNA hybridization and in vitro transcription with nuclear extracts from Xenopus oocytes. Evidence is presented suggesting that tRNA genes are distributed in the rat genome in small clusters spanning 1 to 2 kb and interspersed with large regions (minimum 8 to 20 kb) of non tRNA-coding DNA. The tRNA gene clusters were found to contain the sequences for a variety of tRNA species. Genes for a single isoacceptor, were found in more than one clone. The detailed study of one clone shows the repetition of a cluster of four tRNA sequences at a distance of about 8 kb. The arrangement of tRNA genes in rat appears to follow the irregular pattern of tRNA gene organization previously reported in Drosophila and Xenopus.

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