Open AccessNucleotide sequence of the Aspergillus nidulans mitochondria! gene coding for the small ribosomal subunit RNA: homology to E. coli 16S rRNA
Author(s) -
Heinrich G. Köchel,
Hans Küntzel
Publication year - 1981
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/9.21.5689
Subject(s) - biology , ribosomal rna , genetics , nucleic acid sequence , homology (biology) , gene , microbiology and biotechnology , mitochondrial dna , aspergillus nidulans , mt rnr1 , 5.8s ribosomal rna , rna , non coding rna , mutant
The complete primary structure of the 1437 bp gene coding for mitochondrial 15S rRNA and its flanking regions was determined by Maxam-Gilbert sequencing of cloned HindIII fragment H3 of A. nidulans mtDNA. The gene product reveals significant homology (59%) to E. coli 16S rRNA, and the potential secondary structures of both rRNA molecules are very similar, except that the hairpin structures 7, 8 and 30 of the Brimacombe 16S rRNA model are deleted, and that two sequences of 8 and 31 nucleotides are inserted in the mitochondrial species.