Open AccessThe chemical synthesis of oligoribonucleotides VII. A comparison of condensing agents in the coupling of silybted ribonucleosides
Author(s) -
Kelvin K. Ogilvie,
Richard T. Pon
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.9.2105
Subject(s) - biology , coupling (piping) , combinatorial chemistry , chemical synthesis , function (biology) , nucleic acid , biochemistry , chemistry , materials science , genetics , in vitro , metallurgy
The t-butyldimethylsilyl group is shown to be an ideal protecting group for the 2T-hydroxyl function of ribonucleosides during the synthesis of ribonucleotides using any of nine commonly used condensing agents. The phosphite coupling procedure compares favorably with all of the widely used condensing agents and provides a most convenient route to the key intermediates in the "modified" triester strategy.