Open AccessAntibodies against the subunits of E.coli RNA polymerase as probes for subunit-specific binding of DNA and other ligands
Author(s) -
Walter Stender
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.6.1405
Subject(s) - biology , microbiology and biotechnology , protein subunit , specificity factor , dna , polymerase , rna polymerase , rna , antibody , dna polymerase , rna dependent rna polymerase , virology , genetics , biochemistry , gene
Antibodies against the isolated subunits alpha, beta, and beta' of DNA-dependent RNA polymerase from E. coli have been prepared. They have been used to compare the extent of antibody-binding, as measured by complement fixation, to the isolated subunits and to the intact enzyme, in the absence and presence of ligands, such as inhibitors, nucleotides, nucleosides, oligo- and poly-nucleotides, and DNA of different composition. In many cases the results show a subunit-specific dependence of complement fixation upon the presence of a ligand and suggest a functional topography of the interaction between the subunits alpha, beta, and beta' of RNA polymerase and defined nucleotide sequences and small ligands.