Open AccessComplete nucleotide sequence of a cloned chickena-globin cDNA
Author(s) -
Nicholas J. Deacon,
John Shine,
Honami Naora
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.6.1187
Subject(s) - biology , complementary dna , globin , microbiology and biotechnology , alpha (finance) , pbr322 , nucleic acid sequence , hindiii , genetics , dna , plasmid , gene , medicine , construct validity , nursing , patient satisfaction
Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type clones (pCG alpha-8) was fully sequenced and found to code for neither alpha A- nor alpha D-globin. Partial sequencing of the other alpha-cDNA clones indicates that they are all of the same type.