Complete nucleotide sequence of a cloned chickena-globin cDNA | Zendy

Nicholas J. Deacon | Zendy; John Shine | Zendy; Hiroto Naora | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Complete nucleotide sequence of a cloned chickena-globin cDNA

Author(s) -

Nicholas J. Deacon,

John Shine,

Hiroto Naora

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.6.1187

Subject(s) - biology , complementary dna , globin , microbiology and biotechnology , alpha (finance) , pbr322 , nucleic acid sequence , hindiii , genetics , dna , plasmid , gene , medicine , construct validity , nursing , patient satisfaction

Chicken globin double-stranded cDNA was synthesised from anaemic adult reticulocyte alpha- and beta-globin mRNA and ligated into the Hind III site of pBR322 using synthetic Hind III decamers. Transformation of E. coli x1776 resulted in the production of a number of alpha- and beta-cDNA clones. One of the alpha-type clones (pCG alpha-8) was fully sequenced and found to code for neither alpha A- nor alpha D-globin. Partial sequencing of the other alpha-cDNA clones indicates that they are all of the same type.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research