Open AccessCloning of human immunoglobulin μ gene and comparison with mouse μ gene
Author(s) -
Nobutaka Takahashi,
S. Nakai,
Tasuku Honjo
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.24.5983
Subject(s) - biology , gene , nucleic acid sequence , microbiology and biotechnology , genetics , heteroduplex , molecular cloning , recombinant dna , southern blot , sequence analysis , dna , genomic library , peptide sequence
We have cloned a 12 kb DNA segment containing human mu gene and its flanking sequence from human fetal liver DNA library using mouse mu gene as a probe. Partial nucleotide sequence determination shows that the cloned DNA contains the sequence encoding human mu chain. This is the first constant region gene of the human heavy chain that is cloned. We have compared human and mouse mu genes by heteroduplex analysis and Southern blot hybridization. The results clearly show that not only the sequence encoding the CH4 domain but also the 5'-flanking (S mu) sequence is conserved between human and mouse mu genes, suggesting that the nucleotide sequence in the S mu region has an important biological function, presumably a recognition signal for the class switch recombinant as proposed previously.