Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase | Zendy

Luciano Paolozzi | Zendy; P. Ghelardini | Zendy; Jean-Claude Liébart | Zendy; A. Capozzoni | Zendy; C. Marchelli | Zendy

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Two classes of Mu lig mutants: the thermosensitives for integration and replication and the hyperproducers for ligase

Author(s) -

Luciano Paolozzi,

P. Ghelardini,

Jean-Claude Liébart,

A. Capozzoni,

C. Marchelli

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.23.5859

Subject(s) - biology , dna ligase , replication (statistics) , mutant , dna replication , genetics , dna ligases , microbiology and biotechnology , computational biology , dna , virology , gene

We have previously shown that Mu restores an active DNA replication at non-permissive temperature in E. coli K12 ligts7 strains. In this paper we describe two new types of phage mutants for the Mu lig function. The Mu ligts mutants are conditional lethals defective for both integration and replication of DNA, unable to 'complement' the bacterial lig mutation; the map between B and lys. The mutants of the other type, on the other hand, are able to restore to a maximum level the activity impaired by the ligts7 mutation in the host. We suggest the hypothesis that the product of Mu lig gene could be part of a complex as a topoisomerase.

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