Intervening sequences in a Dictyostelium gene that encodes a low abundance class mRNA | Zendy

Alan R. Kimmel | Zendy; Richard Firtel | Zendy

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Intervening sequences in a Dictyostelium gene that encodes a low abundance class mRNA

Author(s) -

Alan R. Kimmel,

Richard Firtel

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.23.5599

Subject(s) - biology , intron , gene , dictyostelium , rna splicing , genetics , splice site mutation , splice , coding region , messenger rna , nuclease , microbiology and biotechnology , rna

Using S1 nuclease protection experiments and DNA sequencing, we have identified two intervening sequences (introns) within a Dictyostelium gene that codes for a low abundance class mRNA. The two introns are located within the protein coding region of this gene. Both are small (approximately 100 bp) and extremely (approximately 95%) A + T rich. The splice junction sequences are similar to the splice sites in other eukaryotes. Finally, we have shown that these introns are transcribed as part of a higher molecular weight nuclear precursor.

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