The construction of a recombinant cDNA library representative of the po1y(A)+mRNA population from normal human lymphocytes | Zendy

D E Woods | Zendy; Julian M. Crampton | Zendy; Berwyn Clarke | Zendy; Robert Williamson | Zendy

Open Access

The construction of a recombinant cDNA library representative of the po1y(A)⁺mRNA population from normal human lymphocytes

Author(s) -

D E Woods,

Julian M. Crampton,

Berwyn Clarke,

Robert Williamson

Publication year - 1980

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/8.22.5157

Subject(s) - complementary dna , biology , cdna library , recombinant dna , microbiology and biotechnology , rna , plasmid , messenger rna , population , dna , gene , genetics , demography , sociology

A recombinant library has been constructed using the plasmid pAT153 and double stranded cDNA prepared from normal human lymphocyte poly(A)+ RNA. Transformation conditions were optimized to yield approximately 200,000 recombinants per microgram of double stranded cDNA. Statistical analysis as well as sequence complexity analysis of the inserted sequences indicates that the cDNA library is representative of > 99% of the poly(A)+ RNA present in the normal human lymphocyte.

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