Open AccessThe 5′ flanking region of human ε-globin gene
Author(s) -
Francisco E. Baralle,
Carol C. Shoulders,
S. E. Y. Goodbourn,
Alec J. Jeffreys,
Nicholas J. Proudfoot
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.19.4393
Subject(s) - biology , genetics , gene , nucleic acid sequence , dna , 5' flanking region , sequence (biology) , repeated sequence , microbiology and biotechnology , globin , gene cluster , genomic dna , restriction map , sequence analysis , genome , promoter , gene expression
The structural analysis of the 2.0 kb region upstream from the epsilon-globin gene has been carried out. A genomic DNA map around the gene was worked out in some detail to ensure that the cloned DNA was representative of the actual chromosomal arrangement. Furthermore, a new technique was developed to precisely map a reiterated DNA sequence present 1.5 kb to the 5' side of the gene. The complete nucleotide sequence of the 2.0 kb 5' flanking region was then determined and overlapped with the gene. The sequence included the reiterated DNA sequence which is homologous to the so-called AluI family of repeats. Unusual stretches of sequence 50 nucleotides long, where A + T represent about 90% of the bases, are present at both the 5' and 3' sides of the repeat.