Open AccessLocalisation of a series of intra-RNA cross-links in 16S RNA, induced by ultraviolet irradiation of Escberichia coli 30S ribosomal subunits
Author(s) -
Christian W Zwieb,
Richard Brimacombe
Publication year - 1980
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/8.11.2397
Subject(s) - biology , 30s , ribosomal rna , rna , ribosome , ultraviolet irradiation , microbiology and biotechnology , irradiation , genetics , gene , physics , nuclear physics
Mild ultraviolet irradiation of E. coli ribosomal subunits leads to the formation of a number of intra-RNA cross-links, in addition to the RNA-protein cross-links already reported (see refs. 9, 10). After partial ribonuclease digestion of the RNA from irradiated subunits, complexes containing these intra-RNA cross-links can be isolated on a two-dimensional gel electrophoresis system, and subjected to sequence analysis. A series of these cross-linked complexes is described, and the cross-linked RNA regions are compared with the secondary structures derived for 16S RNA (see refs. 6, 7).