Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-C DNA | Zendy

Wing L. Sung | Zendy; Hansen M. Hsiung | Zendy; Roland Brousseau | Zendy; J.J. Michniewicz | Zendy; Ray Wu | Zendy; Saran A. Narang | Zendy

Open Access

Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-C DNA

Author(s) -

Wing L. Sung,

Hansen M. Hsiung,

Roland Brousseau,

J.J. Michniewicz,

Ray Wu,

Saran A. Narang

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.8.2199

Subject(s) - biology , human insulin , dna , oligomer , insulin , sequence (biology) , gene , biochemistry , dna sequencing , combinatorial chemistry , microbiology and biotechnology , organic chemistry , chemistry

The purification of protected deoxyribooligonucleotides containing phosphotriester internucleotidic linkages has been improved by developing a deactivated silica gel chromatographic technique. The efficiency of this technique as applied in the modified phosphotriester approach has been demonstrated in the rapid synthesis of seventeen pure fragments constituting the sequence of human insulin B and mini-C DNA. The sequence of each oligomer was confirmed by the two-dimensional mobility shift method of fingerprinting.

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