Cleavage of single-stranded DNA by the A and A*proteins of bacteriophage φX174 | Zendy

S.A. Langeveld | Zendy; A.D.M. van Mansfeld | Zendy; Jochen Winter | Zendy; Peter Weisbeek | Zendy

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Cleavage of single-stranded DNA by the A and A^*proteins of bacteriophage φX174

Author(s) -

S.A. Langeveld,

A.D.M. van Mansfeld,

Jochen Winter,

Peter Weisbeek

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.8.2177

Subject(s) - biology , dna , bacteriophage , dna replication , microbiology and biotechnology , cleavage (geology) , cleave , dna clamp , biochemistry , escherichia coli , gene , rna , paleontology , reverse transcriptase , fracture (geology)

The purified A protein and A* protein of bacteriophage phi X174 have been tested for endonuclease activity on single stranded viral phi X174 DNA. The A protein (55.000 daltons) nicks single-stranded DNA in the same way and at the same place as it does superhelical RFI DNA, at the origin of DNA replication. The A* protein (37.000 daltons) can cleave the single-stranded viral DNA at many different sites. It has however a strong preference for the origin of replication. Both proteins generate 3'OH ends and blocked 5' termini at the nick site.

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