Open AccessCleavage of single-stranded DNA by the A and A*proteins of bacteriophage φX174
Author(s) -
S.A. Langeveld,
A.D.M. van Mansfeld,
Jochen Winter,
Peter Weisbeek
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.8.2177
Subject(s) - biology , dna , bacteriophage , dna replication , microbiology and biotechnology , cleavage (geology) , cleave , dna clamp , biochemistry , escherichia coli , gene , rna , paleontology , reverse transcriptase , fracture (geology)
The purified A protein and A* protein of bacteriophage phi X174 have been tested for endonuclease activity on single stranded viral phi X174 DNA. The A protein (55.000 daltons) nicks single-stranded DNA in the same way and at the same place as it does superhelical RFI DNA, at the origin of DNA replication. The A* protein (37.000 daltons) can cleave the single-stranded viral DNA at many different sites. It has however a strong preference for the origin of replication. Both proteins generate 3'OH ends and blocked 5' termini at the nick site.