Open AccessThermal perturbation differential spectra of ribonucleic acids. U. Nearest neighbour interactions
Author(s) -
Denise Fréchet,
Ricardo Ehrlich,
Pierre Rémy,
Jacques Gabarro-Arpa
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.7.1981
Subject(s) - stacking , nucleic acid , perturbation (astronomy) , spectral line , normalization (sociology) , dimer , biology , thermodynamics , statistical physics , physics , nuclear magnetic resonance , biochemistry , quantum mechanics , sociology , anthropology
Dinucleoside monophosphates are used here as models for studying sequence dependence of the hypochromic effect correlated with base stacking. It was shown that once the contribution due to the temperature dependent hydration change of the bases is substracted from the thermal perturbation difference spectra of dinucleoside monophosphates, the absorbance change of the dimer only due to unstacking of the bases could be obtained. In order to be able to use these corrected thermal perturbation difference spectra as models for studying nearest neighbour interactions in nucleic acids, it was necessary to normalize them to 100% unstacking of the bases. To perform this normalization, apparent thermodynamic parameters were extracted from the corrected transition curves by means of the two-state model.