Open AccessDetermination of the length distribution of poly(A) at the 3′ terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA
Author(s) -
Paul Ahlquist,
Paul Kaesberg
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.5.1195
Subject(s) - biology , rna ligase , rna , rhinovirus , virology , poliovirus , virus , messenger rna , globin , microbiology and biotechnology , cowpea mosaic virus , population , genetics , plant virus , gene , demography , sociology
Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.