Open AccessMolecular cloning and sequence analysis of adult chicken β globin cDNA
Author(s) -
Robert I. Richards,
John Shine,
Axel Ullrich,
J. R. E. Wells,
Howard M. Goodman
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.5.1137
Subject(s) - biology , complementary dna , globin , cloning (programming) , molecular cloning , genetics , sequence analysis , microbiology and biotechnology , base sequence , sequence (biology) , nucleic acid sequence , gene , computational biology , computer science , programming language
The molecular cloning and nucleotide sequence analysis of adult chicken beta globin mRNA is reported. DNA sequences derived from in vitro transcrption of globin mRNA were purified and amplified as recombinant DNA using the plasmid pBR322. Sequence analysis of several clones coding for beta globin strongly suggests that transcription errors may be generated near the 5' end of transcripts in vitro by reverse transcription. The complete sequence of the longest beta globin insert containing 51 bases of the 5' untranslated region as well as the complete coding and 3' untranslated regions has been determined.