A nuclear precursor to human γ-globin messenger RNA | Zendy

Michael Courtney | Zendy; R. Williamson | Zendy

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A nuclear precursor to human γ-globin messenger RNA

Author(s) -

Michael Courtney,

R. Williamson

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/7.5.1121

Subject(s) - biology , polyadenylation , microbiology and biotechnology , messenger rna , rna , globin , complementary dna , transcription (linguistics) , recombinant dna , post transcriptional modification , gene , rna editing , biochemistry , linguistics , philosophy

RNA isolated from human foetal liver at an erythroid stage of development was transferred from methyl mercury hydroxide agarose gels to diazobenzyloxymethyl paper. A 16S RNA species containing gamma-globin mRNA sequences was identified by hybridisation to 32P-labelled human gamma-globin cDNA recombinant plasmid pHgamma GI. This RNA is found only in the cell nucleus and is polyadenylated. Human globin gene transcription is thus analagous to that in mouse: interventing sequences are transcribed and processed from the pre-mRNA in the nucleus after polyadenylation.

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