Open AccessA nuclear precursor to human γ-globin messenger RNA
Author(s) -
Michael Courtney,
Robert Williamson
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.5.1121
Subject(s) - biology , polyadenylation , microbiology and biotechnology , messenger rna , rna , globin , complementary dna , transcription (linguistics) , recombinant dna , post transcriptional modification , gene , rna editing , biochemistry , linguistics , philosophy
RNA isolated from human foetal liver at an erythroid stage of development was transferred from methyl mercury hydroxide agarose gels to diazobenzyloxymethyl paper. A 16S RNA species containing gamma-globin mRNA sequences was identified by hybridisation to 32P-labelled human gamma-globin cDNA recombinant plasmid pHgamma GI. This RNA is found only in the cell nucleus and is polyadenylated. Human globin gene transcription is thus analagous to that in mouse: interventing sequences are transcribed and processed from the pre-mRNA in the nucleus after polyadenylation.