Open AccessA new series of RNAs associated with the genome of spleen focus fonning virus (SFFV) and poly(A)-containing RNA from SFFV-infected cells
Author(s) -
Fumio Harada,
Yoji Ikawa
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.4.895
Subject(s) - biology , rna , rnase p , virus , oligonucleotide , virology , microbiology and biotechnology , genome , nucleic acid thermodynamics , messenger rna , microrna , genetics , dna , gene
A series of low molecular weight RNAs (4.5 to 5.5S) as well as other 4 to 7S RNAs were dissociated from genomic RNA of spleen focus forming virus (SFFV) by heating. On two dimensional polyacrylamide gel electrophoresis, this series of RNAs gave a series of more than thirty spots. RNase T1 fingerprints of these spots were identical except for differences in 3'-terminal oligonucleotides, which were mainly due to different numbers of uridylic acid residues, larger RNA-molecules containing poly(U)sequences at their 3'-termini. This series of RNAs is also associated with poly(A)-containing nuclear and cytoplasmic RNAs from SFFV-infected cells.