Open AccessDetermination of base pairing in Escherichia coil and Bacillus stearothermophilus 5S RN As by infrared spectroscopy
Author(s) -
Bernd Appel,
Volker A. Erdmann,
Josef Stulz,
Th. Ackermann
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/7.4.1043
Subject(s) - biology , pairing , escherichia coli , base pair , base (topology) , bacillaceae , bacillus (shape) , infrared , genetics , physics , bacteria , bacillus subtilis , dna , gene , optics , mathematical analysis , superconductivity , mathematics , quantum mechanics
The extent of base pairing in Escherichia coli and Bacillus stearothermophilus 5S RNAs was determined by infrared spectroscopy. From the infrared spectra taken at 20 degrees and 52 degrees C it is concluded that E. coli and B. stearothermophlius 5S RNAs possess a large number of base pairs (Table I). Comparison of our results with those previously published using other methods leads to the conclusion that the structures of prokaryotic 5S RNAs involve a large number of tertiary interactions, in which the base pairing is not necessarily solely of the Watson-Crick type.