Open AccessNuckotide sequence of the three major early promoters of bacteriophage T7
Author(s) -
Ulrich Siebenlist
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/6.5.1895
Subject(s) - biology , promoter , rna polymerase , bacteriophage , genetics , homology (biology) , t7 rna polymerase , polymerase , consensus sequence , nucleic acid sequence , microbiology and biotechnology , rna , dna , gene , base sequence , escherichia coli , gene expression
I have determined the nucleotide sequences of the three major early promoters of bacteriophage T7 (A1, A2, A3). The sequences confirm the two main homologies found between other known promoters for E. coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyl transferase, E.C. 2. 7. 7. 6). In particular, all three T7 promoters show a very good match with the -35 region homology; the A2 and A3 promoters share a 17 basepair sequence in this region. On the other hand, the match with the Pribnow Box homology is much less pronounced and different for each T7 promoter.