RNA-protein cross-linking in Escherichia coli 30s ribosomal subunits: precise localisation of the nucleotide in 16s RNA which is coupled to protein S7 by ultraviolet irradiation | Zendy

Christian Zwieb | Zendy; Richard Brimacornbe | Zendy

Open Access

RNA-protein cross-linking in Escherichia coli 30s ribosomal subunits: precise localisation of the nucleotide in 16s RNA which is coupled to protein S7 by ultraviolet irradiation

Author(s) -

Christian Zwieb,

Richard Brimacornbe

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/6.5.1775

Subject(s) - biology , 30s , ribosomal rna , rna , nucleic acid , escherichia coli , ribosomal protein , ribosome , nucleotide , microbiology and biotechnology , genetics , gene

It is well established that when E. coli 30S ribosomal subunits are irradiated with ultraviolet light under mild conditions a specific cross-link is formed between protein S7 and the 16S RNA. Methodology is presented for the analysis of the single nucleotide residue concerned in this cross-link. Firstly, the identity of the ribonuclease T1 octanucleotide attached to S7 is confirmed by a new method, which involves isolation and analysis of S7-polynucleotide complexes containing 30 -- 40 nucleotides. Secondly, the isolated S7-octanucleotide complex is digested successively with ribonuclease A, proteinase K and ribonuclease T2, and the nucleotides liberated are identified. The results show unambiguously that uridine residue number 1239 in the 16S RNA sequence is cross-linked to protein S7.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research