Open AccessDNA polymerase α, β and γ activities in ultraviolet irradiated CV-1 monkey cells
Author(s) -
R. Wicker,
Anna Ivana Scovassi,
S. Nocentini
Publication year - 1979
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/6.4.1591
Subject(s) - biology , sucrose gradient , dna polymerase , microbiology and biotechnology , dna , polymerase , ultraviolet light , centrifugation , ultraviolet irradiation , irradiation , ultraviolet , enzyme , sucrose , fractionation , biochemistry , dna repair , chromatography , chemistry , physics , photochemistry , quantum mechanics , nuclear physics
To determine the possible role of DNA polymerase alpha, beta and gamma during the repair period following ultraviolet (lambda max : 254 nm) irradiation of monkey CV-1 cells, we measured the three enzymatic activities by using specific tests, either in crude extracts or after fractionation by sucrose gradient (5--20%) centrifugation at high salt concentration. When compared to the unirradiated control, we could not detect any significant variation in the levels of activity of DNA polymerases alpha, beta and gamma at any time (0, 12 to 48 h) after ultraviolet irradiation of the cells with doses ranging from 9 to 52.5 J.m-2.