Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance | Zendy

Pari Davanloo | Zendy; Mathias Sprinzl | Zendy; Kimitsuna Watanabe | Zendy; Martin Albani | Zendy; Helga Kersten | Zendy

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Role of ribothymidine in the thermal stability of transfer RNA as monitored by proton magnetic resonance

Author(s) -

Pari Davanloo,

Mathias Sprinzl,

Kimitsuna Watanabe,

Martin Albani,

Helga Kersten

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/6.4.1571

Subject(s) - transfer rna , biology , uridine , mutant , rna , methionine , biochemistry , nuclear magnetic resonance spectroscopy , stereochemistry , crystallography , chemistry , amino acid , gene

In order to elucidate the functional role of the modified uridines at position 54 of tRNA, the 270 MHz high-field proton NMR spectra of methionine tRNAs from E. coli, from a mutant thereof, and from T. thermophilus, containing ribothymidine, uridine and 2-thioribothymidine, respectively, have been measured as a function of temperature. A comparison of the NMR melting profiles of the minor nucleosides from these tRNAs shows that the melting temperature of uridine containing tRNA is 6 degrees C lower than that of the wild type tRNA whereas that of the 2-thioribothymidine tRNA is 7 degrees C higher than that of the wild type tRNA. These results, therefore, demonstrate that these modifications serve for stabilization of the tertiary structure of tRNA.

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