A precise quantitation of gene number by saturation hybridization using cloned DNA | Zendy

Shirley Longacre | Zendy; Bernard Mach | Zendy

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A precise quantitation of gene number by saturation hybridization using cloned DNA

Author(s) -

Shirley Longacre,

Bernard Mach

Publication year - 1979

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/6.4.1241

Subject(s) - biology , complementary dna , microbiology and biotechnology , globin , gene , nucleic acid thermodynamics , dna , genetics , hybridization probe , rna

This paper describes a precise method of gene titration as applied to the alpha- and beta-globin genes in the mouse. The three salient features of the method are: (i) the use of saturation hybridization in probe cDNA excess, (ii) the use of highly purified cDNA probes prepared by preparative hybridization with cloned globin sequences (Longacre and Mach (1978) J. Biol. Chem. 253, 7500) and (iii) the use of cloned globin sequences to calibrate the system internally. The results indicate that there are two genes for alpha-globin and two genes for beta-globin in the BALB/c mouse. The significance of these results are discussed in relation to other data regarding adult and embryonic globin genes.

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