Differential susceptibilities of DNA polymerases-αand -βto polyanions | Zendy

Takashi Shimada | Zendy; Michiyuki Yamada | Zendy; Masanao Miwa | Zendy; Hiroshi Nagano | Zendy; Yoshitake Mano | Zendy

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Differential susceptibilities of DNA polymerases-αand -βto polyanions

Author(s) -

Takashi Shimada,

Michiyuki Yamada,

Masanao Miwa,

Hiroshi Nagano,

Yoshitake Mano

Publication year - 1978

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/5.9.3427

Subject(s) - biology , dna , dna polymerase , polymerase , genetics , differential (mechanical device) , nucleotidyltransferase , base sequence , computational biology , rna , gene , physics , thermodynamics

The effects of various polyanions including synthetic polynucleotides on DNApolymerases-alpha and -beta from blastulae of the sea urchin Hemicentrotus pulcherrimus and HeLa cells were studied. Only DNA polymerase-alpha was inhibited by polyanions, such as polyvinyl sufate, dextran sulfate, heparin, poly(G), poly(I), poly(U) and poly(ADP-Rib). Of the various polynucleotides tested, poly(G) and poly(I) were the strongest inhibitors. Kinetic studies showed that the Ki value for poly(G) was 0.3 microgram/ml and that poly(G) had 20-fold higher affinity than activated DNA for the template-primer site of DNA polymerase-alpha. Poly(U) and poly(ADP-Rib) were also inhibitory, but they were one hundredth as inhibitory as poly(G) or poly(I). Poly(A), poly(C), poly(A).poly(U) AND POLY(I).poly(C) were not inhibitory to DNA polymerase-alpha. In contrast, DNA olymerase-beta was not affected at all by these polyanions under the same conditions.

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