Open AccessDistribution of 7-methylguanine and of replication sites in the different kinetic classes of DNA from rats treated with dimethyhtitrosamine
Author(s) -
A.I. Galbraith,
Marilyn R. Chapleo,
Ruth F. Itzhaki
Publication year - 1978
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/5.9.3357
Subject(s) - biology , chromatin , dna , dna replication , thymidine , dna synthesis , microbiology and biotechnology , genetics
The distribution of 7-methylguanine in the families of repetitive and unique sequences of rat liver chromatin DNA has been studied using the technique of DNA-DNA reassociation. Rats were injected with di[14C]methylnitrosamine and chromatin DNA was prepared 3 h later. The distribution of 7-methylguanine was found to be random between these classes of DNA. We have also studied chromatin DNA from rats treated with unlabelled DMN plus [3H]thymidine in this way, in order to find if DMN affects DNA synthesis within any one kinetic class. Our results suggest that there is no difference in the extent of synthesis between these classes.