Open AccessSequence organization of the β-globin mRNA precursor
Author(s) -
Kate Smith,
Jerry B. Lingrel
Publication year - 1978
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/5.9.3295
Subject(s) - biology , globin , messenger rna , complementary dna , rnase p , microbiology and biotechnology , gene , sequence (biology) , rna , genetics
The sequence organization of the beta-globin mRNA precursor has been determined directly by analyzing the resistant fragments from the RNase A digestion of the precursor RNA-globin cDNA hybrid. Three fragments are obtained which proves that the beta-globin mRNA sequence in its precursor is split into three discontinuous segments. The two intervening sequences in the beta-globin gene are therefore transcribed and removed during mRNA maturation.