Interaction of Escherichia coli glutaminyl-tRNA synthetase with noncognate tRNA's | Zendy

Tetsuzo Seno | Zendy; A. Nakamura | Zendy; Shoji Fukuhara | Zendy; Kimiko Iwata | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Interaction of Escherichia coli glutaminyl-tRNA synthetase with noncognate tRNA's

Author(s) -

Tetsuzo Seno,

A. Nakamura,

Shoji Fukuhara,

Kimiko Iwata

Publication year - 1978

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/5.5.1561

Subject(s) - transfer rna , escherichia coli , biology , biochemistry , glutamine , enzyme , glutamine synthetase , microbiology and biotechnology , amino acid , rna , gene

Several noncognate tRNA's from Escherichia coli were mischarged with glutamine by E. coli glutaminyl-tRNA synthetase if dimethylsulfoxide was present in the reaction mixture. Kinetic analysis of the mischarging revealed that dimethyl sulfoxide stimulated the misacylation by affecting the maximum velocity. Several noncognate tRNA's were shown to interact with glutaminyl-tRNA synthetase as measured by their ability to protect the enzyme against thermal inactivation or to replace cognate tRNA in stimulating glutamine-dependent ATP-PPi exchange reaction. These tRNA's, however, did not coincide with those which were mischargeable with glutamine.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research