Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S3cells | Zendy

Siegfried Detke | Zendy; Janet L. Stein | Zendy; Gary S. Stein | Zendy

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Synthesis of histone messenger RNAs by RNA polymerase II in nuclei from S phase HeLa S₃cells

Author(s) -

Siegfried Detke,

Janet L. Stein,

Gary S. Stein

Publication year - 1978

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/5.5.1515

Subject(s) - biology , microbiology and biotechnology , rna polymerase ii , transcription (linguistics) , histone h2a , histone , polymerase , rna , messenger rna , rna polymerase iii , rna polymerase i , rna polymerase , gene expression , gene , biochemistry , promoter , linguistics , philosophy

Nuclei were isolated from synchronized HeLa S3 cells and transcribed utilizing their endogenous RNA polymerases. Our data suggest that S phase nuclei are capable of synthesizing histone mRNA sequences while nuclei from G1 phase cells are not. Transcription of histone mRNA sequences by S phase nuclei can be abolished completely by low levels of alpha-amanitin (1.0 microgram/ml, a concentration which completely inhibits RNA polymerase II). From these results it appears that transcription of the histone mRNA sequences occurs during the S phase but not during the G1 phase of the cell cycle and that RNA polymerase II is responsible for histone gene readout.

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