Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site | Zendy

David V. Goeddel | Zendy; Daniel G. Yansura | Zendy; Marvin H. Caruthers | Zendy

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Studies on gene control regions. VI. The 5- methyl of thymine, a lac repressor recognition site

Author(s) -

David V. Goeddel,

Daniel G. Yansura,

Marvin H. Caruthers

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.9.3039

Subject(s) - thymine , biology , lac repressor , repressor , uracil , binding site , dna , gene , genetics , microbiology and biotechnology , stereochemistry , gene expression , chemistry

Three site specific deoxyuridine analogs of lac operator were tested for binding with wild type (SQ) and tight binding (QX86) lac repressors. Insertion of uracil for thymine at site 13 (our nomenclature) significantly reduced the dissociation half-life of QX86 repressor for lac operator DNA (21 vs 1.2 min). Two other sites (6 and 7) are affected to a much lesser extent.

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