The specificity of S1nuclease toward RNA-DNA hybrids as studied using isotopes of phosphorus-32 and phosphorus-33 | Zendy

Scott C. Wittelsberger | Zendy; J N Hansen | Zendy

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The specificity of S₁nuclease toward RNA-DNA hybrids as studied using isotopes of phosphorus-32 and phosphorus-33

Author(s) -

Scott C. Wittelsberger,

J N Hansen

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.6.1829

Subject(s) - nuclease , rna , biology , dna , ribonuclease , bacillus cereus , transfer rna , ribosomal rna , hybrid , phosphorus , biochemistry , microbiology and biotechnology , genetics , bacteria , botany , chemistry , organic chemistry , gene

Hybrids were formed from Bacillus cereus DNA and ribosomal RNA. They were treated with various combination of S1 nuclease and ribonuclease, and the molar ratios of the RNA and DNA moieties remaining in the treated hybrids were determined using a 32P-33P dual-label technique. It was found that both S1 nuclease and ribonuclease are required to give hybrid with RNA and DNA in a perfect 1:1 molar ratio. It was noted that the dual-label technique which employs orthophosphate as the sole phosphorus source for both labels gives unambiguous molar ratios and obviates the need to calculate specific activities, make quench corrections, or correct for base content.

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