Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger | Zendy

Olivier Destrée | Zendy; AnneLise Haenni | Zendy; Max L. Birnstiel | Zendy

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Histone mRNA in Xenopus laevis ovaries: identification of the H4 messenger

Author(s) -

Olivier Destrée,

AnneLise Haenni,

Max L. Birnstiel

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.4.801

Subject(s) - biology , xenopus , microbiology and biotechnology , messenger rna , rna , histone h4 , histone , dna , gene , biochemistry

RNA extracted from ovaries of adult Xenopus laevis and introduced into a wheat germ cell-free system, directs the synthesis of polypeptides co-electrophoresing with all five histones. By frationation of total ovary RNA through Sephadex G-200 and polyacrylamide gel, RNA was isolated that is translated into histones 4 (H4) only. In addition, this RNA labelled in vitro with [I125] hybridizes to a restriction fragment of cloned sea urchin DNA known to contain the H4 gene.

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