Synthesis of oltgonucleotides with sequences identical with or analogous to the 3′-end of 16S ribosomal RNA of Es cherichia coli: preparation of m62A-C-C-U-C-C and A-C-C-U-C-m42C via phosphotriester intermediates1 | Zendy

Jacques H. van Boom | Zendy; Peter Burgers | Zendy; Roberto Crea | Zendy; G. van der Marel | Zendy; G. Wille | Zendy

Open Access

Synthesis of oltgonucleotides with sequences identical with or analogous to the 3′-end of 16S ribosomal RNA of Es cherichia coli: preparation of m⁶₂A-C-C-U-C-C and A-C-C-U-C-m⁴₂C via phosphotriester intermediates¹

Author(s) -

Jacques H. van Boom,

Peter Burgers,

Roberto Crea,

G. van der Marel,

G. Wille

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.3.747

Subject(s) - biology , ribosomal rna , rna , stereochemistry , block (permutation group theory) , biochemistry , microbiology and biotechnology , gene , combinatorics , chemistry , mathematics

The synthesis of two fully-protected hexanucleotides (11a and 11b) via a phosphotriester approach, which is based on the use of two types of protecting groups for the internucleotide linkages, i.e. one 2,2,2-tribromo-ethyl at the 5'-terminus and four 2-chlorophenyl groups for the remaining linkages, is reported. The hexanucleotides 11a and 11b, assembled via a block-wise two-step phosphotriester method, can be deblocked conveniently to give the two hexamers 12a and 12b containing only 3'leads to5' internucleotide linkages.

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