Open AccessTranscriptional organization of the 5.8S ribosomal RNA ristron in Xenopus laevis ribosomal DNA
Author(s) -
Thomas A. Walker,
Norman R. Pace
Publication year - 1977
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/4.3.595
Subject(s) - biology , cistron , ribosomal rna , ribosomal dna , 28s ribosomal rna , 5s ribosomal rna , xenopus , 18s ribosomal rna , spacer dna , microbiology and biotechnology , ecori , 5.8s ribosomal rna , internal transcribed spacer , genetics , ribosome , dna , rna , gene , restriction enzyme , phylogenetics
Hybridization of purified, 32p-labeled 5.8S ribosomal RNA from Xenopus laevis to fragments generated from X. laevis rDNA by the restriction endonuclease, EcoRI, demonstrates that the 5.8S rRNA cistron lies within the transcribed region that links the 18S and 28S rRNA cistrons.