Isolation and characterization of a spacerless dinucleosome from Hl-depleted chromatin | Zendy

Leonard Klevan | Zendy; Donald M. Crothers | Zendy

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Isolation and characterization of a spacerless dinucleosome from Hl-depleted chromatin

Author(s) -

Leonard Klevan,

Donald M. Crothers

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.12.4077

Subject(s) - micrococcal nuclease , nucleosome , biology , chromatin , dna , histone , base pair , nuclease , microbiology and biotechnology , biophysics , genetics

Calf thymus chromatin, depleted in histone H1, was digested with micrococcal nuclease and fractionated by column chromatography. 140 base pair nucleosome core particles were isolated along with an unusual particle containing 2 histone octamers and 240 base pairs of DNA. Evidence is presented that the spacer DNA region is absent from these modified dinucleosomes, which appear as stable products of the digestion process. The physical properties of both particles are presented along with brief speculation on their possible origin and function.

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