A simple method for the preparation of (β-32P)purine nucleoside triphosphates | Zendy

Yasuhiro Furuichi | Zendy; Aaron J. Shatkin | Zendy

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A simple method for the preparation of (β-³²P)purine nucleoside triphosphates

Author(s) -

Yasuhiro Furuichi,

Aaron J. Shatkin

Publication year - 1977

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/4.10.3341

Subject(s) - deoxyribonucleoside , guanosine , adenylate kinase , nucleoside , biochemistry , biology , ribonucleoside , nucleoside diphosphate kinase , purine , nucleotide , pyruvate kinase , enzyme , purine nucleoside phosphorylase , guanine , guanosine triphosphate , gtp' , rna , glycolysis , gene

A rapid, simple and inexpensive procedure is described for the preparation of purine ribo-and deoxyribonucleoside triphosphates specifically and highly radiolabeled with [32P]phosphate in the beta position. The method involves two successive enzymatic reactions: conversion of donor [gamma-32P]ATP in the presence of an excess of acceptor 5'-mononucleotide to the 5'-diphosphates by myokinase or guanosine 5'-monophosphate kinase followed by phosphorylation with pyruvate kinase to yield 5'-triphosphates.

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