Base composition heterogeneity of mammalian DNAs in CsCl-netropsin density gradient. | Zendy

T. Guttann | Zendy; Hana Votavová | Zendy; L. Pivec | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Base composition heterogeneity of mammalian DNAs in CsCl-netropsin density gradient.

Author(s) -

T. Guttann,

Hana Votavová,

L. Pivec

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.3.835

Subject(s) - netropsin , biology , density gradient , ionic strength , homogeneous , centrifugation , dna , biochemistry , chemistry , physics , thermodynamics , quantum mechanics , minor groove , aqueous solution

DNAs of 15 mammals and some lower organisms were analysed by CsCl-netropsin density gradient centrifugation. Increased resolving power of this method enabled to detect many new components in mammalian DNAs. Distinct components were detected in the density range of the main band. These components found in different mammalian DNAs have probably limited variation in the G+C content. Most of other components seems to be species specific. The DNAs of lower organisms form homogeneous band even in the presence of netropsin. The relation between densities in CsCl-netropsin and CsCl density gradient is nonlinear. This result supports a hypothesis that in high ionic strength netropsin is preferentially bound to (dA.dT) clusters.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research