Open AccessBasic polypeptides as histone models. Effect of conformation, base composition and methylation of nucleic acids on the interaction with Hl and histone models and on the circular dichroism of complexes.
Author(s) -
J. Šponar,
J. Doskočil,
K. Bláha,
Ch. Zimmer
Publication year - 1976
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/3.2.507
Subject(s) - dna , histone , circular dichroism , nucleic acid , biology , rna , dna methylation , base pair , biochemistry , methylation , nucleic acid thermodynamics , microbiology and biotechnology , stereochemistry , biophysics , crystallography , chemistry , gene , gene expression , base sequence
Interaction of histone H 1 and models simulating histone chains was followed by monitoring the melting curves of supernatants after the sedimentation of aggregated complexes. In a mixture of two DNAs the histones reacted selectively with (A+T)-rich and non-methylated DNA, respectively. H 1 and (Ala-Lys-Pro)n also interacted preferentially with DNA in a mixture with double stranded RNA whereas (Lys30,Ala70)n did not show any selectivity. (G+C)-rich DNA in complexes showed CD spectra the intensity of which decreased with increasing DNA methylation to values comparable with these of complexes of (A+T)-rich DNA. In complexed with double stranded RNA only the polymer (Lys30,Ala70) displayed CD pattern similar to spectra of complexes with DNA. It was concluded that formation and structure of complexes depend selectively on the DNA conformation and base composition.