Open AccessComparison of the fine structure of mitochondrial DNA from Saccharomyces cerevisiae and S. carisbergensis: electron microscopy of partially denatured molecules.
Author(s) -
Gunna Christiansen,
Claus Christiansen
Publication year - 1976
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/3.2.465
Subject(s) - biology , ecori , saccharomyces cerevisiae , mitochondrial dna , dna , denaturation (fissile materials) , nucleic acid denaturation , electron microscope , saccharomyces , gel electrophoresis , homology (biology) , restriction enzyme , microbiology and biotechnology , biochemistry , endonuclease , electrophoresis , yeast , base sequence , gene , chemistry , physics , nuclear chemistry , optics
Denaturation-maps of mitochondrial DNA from Saccharomyces cerevisiae and S. carlsbergensis have been derived from electron microscopic observations of partially denatured complete circular molecules and large fragments of these circles. The mitochondrial DNA from the two species differ by 6% in total length, but seems from the maps to contain some regions of apparent close homology. The cleavage pattern of the two DNAs by the restriction endonuclease EcoRI is compared by gel electrophoresis.