Repeating restriction fragments of human DNA | Zendy

Laura Manuelidis | Zendy

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Repeating restriction fragments of human DNA

Author(s) -

Laura Manuelidis

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.11.3063

Subject(s) - biology , dna , base pair , restriction fragment , dimer , microbiology and biotechnology , nucleic acid thermodynamics , genetics , genome , human genome , fragment (logic) , gene , base sequence , chemistry , computer science , programming language , organic chemistry

Human DNA digested with Hae III showed multiple repeats of a 170 base pair fragment. The most prominent band was the 340 base pair dimer, estimated to be 0.8% of the entire genome. Eco R1 and Hha I yielded fragments with similar electrophoretic mobility to the Hae III dimer. In each case this band was markedly enriched in DNA reassociating at a 0t of less than or equal to 1. Hybridization of the Hae III dimer to gels eluted on to filters demonstrated that the multiple Hae III fragments and Eco R1 fragments contained compatible sequences. These sequences may comprise a distinct subclass of DNA.

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