Cleavage of adeno-associated virus DNA with SalI, PstI and HaeII restriction endonucleases | Zendy

Luis M. de la Maza | Zendy; B. J. Carter | Zendy

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Cleavage of adeno-associated virus DNA with SalI, PstI and HaeII restriction endonucleases

Author(s) -

Luis M. de la Maza,

B. J. Carter

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.10.2605

Subject(s) - psti , restriction enzyme , biology , cleavage (geology) , hindiii , dna , restriction map , microbiology and biotechnology , restriction fragment , restriction site , genome , genetics , bamhi , plasmid , gene , paleontology , fracture (geology)

Duplex AAV-2 DNA was digested with SalI, PstI or HaeII restriction endonucleases and the cleavage sites were mapped. SalI cleaves AAV DNA at 0.310 map units, PstI at 0.106, 0.422 and 0.914 and the five HaeII sites were mapped at 0.110. 0.156, 0.181, 0.536 and 0.600 map units. These cleavage products will be useful for the isolation of specific regions from the AAV DNA, located outside of the stably transcribed region of the genome, and will also help to map more complex restriction enzyme cleavages.

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