Open AccessConstruction of a hybrid col El plasmid carrying the gene for bacteriophage repressor
Author(s) -
L. P. Tikhomirova,
Alexander S. Solonin,
V. N. Ksenzenko,
N.I. Matvienko
Publication year - 1976
Publication title -
nucleic acids research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 9.008
H-Index - 537
eISSN - 1362-4954
pISSN - 0305-1048
DOI - 10.1093/nar/3.10.2485
Subject(s) - biology , bacteriophage , plasmid , repressor , genetics , gene , gene expression , escherichia coli
A biologically active hybrid DNA molecule was constructed from plasmid Col E1 and the Eco R1 fragment of lambda DNA containing the gene for lambda repressor. The presence of this gene in the hybrid molecule was demonstrated genetically. The hybrid plasmid contains two closely located targets for restriction endonuclease Hind 111 in the integrated fragment. Thus, the plasmid may be used as a vector not only for Eco R1 fragments but also for Hind 111 fragments.