Induced formation of covalent bonds between nucleoprotein components{middle dot} V{middle dot} UV or bisulfite induced polynucleotide-protein crosslinkage in bacteriophage MS2 | Zendy

E.I. Budowsky | Zendy; N.A. Simukova | Zendy; M.F. Turchinsky | Zendy; Irina V. Boni | Zendy; Yu. M. Skoblov | Zendy

Open Access

Induced formation of covalent bonds between nucleoprotein components{middle dot} V{middle dot} UV or bisulfite induced polynucleotide-protein crosslinkage in bacteriophage MS2

Author(s) -

E.I. Budowsky,

N.A. Simukova,

M.F. Turchinsky,

Irina V. Boni,

Yu. M. Skoblov

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.1.261

Subject(s) - polynucleotide , biology , nucleoprotein , covalent bond , cytosine , nucleic acid , bacteriophage , bisulfite , dna , lysine , deoxyadenosine , biochemistry , rna , amino acid , nucleotide , escherichia coli , stereochemistry , chemistry , organic chemistry , gene expression , gene , dna methylation

UV (lambda = 254 nm) irradiation of bacteriophage MS2 or its treatment with bisulfite induce covalent crosslinkage of the RNA to the coat protein. epilsonN-(2-oxopyrimidyl-4)-lysine was found in the phage hydrolysates after either type of treatment. An equimolar mixture of 0-methylhydroxylamine and bisulfite causes complete disappearance of the cross-links. This led to the conclusion that one of the factors responsible for the UV-induced polynucleotide-protein crosslinkage and the main factor in treatment with bisulfite is substitution of the exocyclic amino group of the activated cytosine nucleus by the lysine residue epilson-amino group of the protein.

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