Studies on the low molecular weight RNA associated with 28S ribosomal RNA from Crotalus durissus terrificus liver | Zendy

J. F. Giorgini | Zendy; Fernando Luiz De Lucca | Zendy

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Studies on the low molecular weight RNA associated with 28S ribosomal RNA from Crotalus durissus terrificus liver

Author(s) -

J. F. Giorgini,

Fernando Luiz De Lucca

Publication year - 1976

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/3.1.165

Subject(s) - biology , rna , ribosomal rna , 28s ribosomal rna , microbiology and biotechnology , ribosome , formamide , 5s ribosomal rna , biochemistry , genetics , gene

A low molecular weight RNA was released from the purified rattlesnake 28 S RNA by brief heat treatment as well as by treatment with 80% dimethylsulfoxide or formamide. The sedimentation coeficient of this low molecular weight RNA was found to be 5.5 S, corresponding to a nucleotide number of 140 and a molecular weight of 46 000. It was also observed that 5.5S RNA is present in equimolar ratio to 5 S rRNA. Heat treatment of the purified 60 S ribosomal subunit also released the 5.5 S RNA. The possibility that this low molecular weight RNA is located on the surface of the large ribosomal subunit is discussed.

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