Alkaline fixation drastically improves the signal of in situ hybridization | Zendy

Eugénia Basyuk | Zendy

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Alkaline fixation drastically improves the signal of in situ hybridization

Author(s) -

Eugénia Basyuk

Publication year - 2000

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/28.10.e46

Subject(s) - fixative , biology , in situ hybridization , in situ , fixation (population genetics) , rna , hybridization probe , microbiology and biotechnology , nucleic acid thermodynamics , molecular probe , dna , biochemistry , messenger rna , gene , cytoplasm , physics , meteorology

In situ hybridization (ISH) is widely used to detect DNA and RNA sequences within the cell and tissue sections. The important step in performing this technique is tissue fixation. We investigated the influence of the pH of the fixative on the outcome of ISH. Our studies indicate that alkaline formaldehyde dramatically increases the ISH signal with RNA probes. The increase in signal was observed for detection of low as well as for high abundance messages. The sensitivity of the method was increased 5- to 6-fold.

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