Fluorescence Detection of Specific Sequence of Nucleic Acids by Oxazole Yellow-Linked Oligonucleotides. Homogeneous Quantitative Monitoring of In Vitro Transcription | Zendy

Takahiko Ishiguro | Zendy; J. Saitoh | Zendy; Hideo Yawata | Zendy; Masami Otsuka | Zendy; Teruhiko Inoue | Zendy; Y. SUGIURA | Zendy

Open Access

Fluorescence Detection of Specific Sequence of Nucleic Acids by Oxazole Yellow-Linked Oligonucleotides. Homogeneous Quantitative Monitoring of In Vitro Transcription

Author(s) -

Takahiko Ishiguro,

J. Saitoh,

Hideo Yawata,

Masami Otsuka,

Teruhiko Inoue,

Y. SUGIURA

Publication year - 1996

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/24.24.4992

Subject(s) - oligonucleotide , biology , rna , nucleic acid , dna , transcription (linguistics) , microbiology and biotechnology , in vitro , nucleotide , footprinting , biochemistry , gene , base sequence , linguistics , philosophy

We have developed a fluorescent DNA probe, oxazole yellow (YO)-linked oligonucleotide complementary to a target DNA/RNA, which can enhance the fluorescence on hybridizing with a target nucleotide. We demonstrated the applicability of the YO-linked oligonucleotide probe to real-time monitoring of the in vitro transcription process of a plasmid DNA constructed containing the 5'-terminus non-coded region of hepatitis C virus RNA. In the process of in vitro transcription in the presence of YO-linked complementary oligonucleotide, the fluorescence of the reaction mixture showed a time-dependent linear increase corresponding to the generated target RNA product.

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