Successful transformation of yeast mitochondria withRPM1: an approach forin vivostudies of mitochondrial RNase P RNA structure, function and biosynthesis | Zendy

Pavol Sulo | Zendy; Kathleen R. Groom | Zendy; Carol A. Wise | Zendy; Marlene C. Steffen | Zendy; Nancy Martín | Zendy

Open Access

Successful transformation of yeast mitochondria withRPM1: an approach forin vivostudies of mitochondrial RNase P RNA structure, function and biosynthesis

Author(s) -

Pavol Sulo,

Kathleen R. Groom,

Carol A. Wise,

Marlene C. Steffen,

Nancy Martín

Publication year - 1995

Publication title -

nucleic acids research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 9.008

H-Index - 537

eISSN - 1362-4954

pISSN - 0305-1048

DOI - 10.1093/nar/23.5.856

Subject(s) - rnase mrp , biology , rnase p , rnase h , mitochondrial dna , mitochondrion , transfer rna , rna , gene , rnase ph , ribonuclease iii , transformation (genetics) , biochemistry , microbiology and biotechnology , genetics , rna interference

Mitochondrial RNase P RNA (Rpm1r) is coded by the RPM1 gene of mitochondrial DNA in many yeasts. As an initial step to developing a genetic approach to the structure and biogenesis of yeast mitochondrial RNase P, biolistic transformation has been used to introduce wild type and altered RPM1 genes into strains containing no mitochondrial DNA. The introduced wild type gene does support RNase P activity demonstrating that pre-existing RNase P activity is not necessary for the biosynthesis of the enzyme. Mutations introduced into RPM1 in vitro result in reduced accumulation of mature tRNA and in an alteration of the processing of Rpm1r in vivo.

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